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Search Completed | Title | Supercritical CO2 extraction of mentha (Mentha piperita L.) at different solvent densities
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Text | Supercritical CO2 extraction of mentha (Mentha piperita L.) at different solvent densities | 001
J. Serb. Chem. Soc. 74 (4) 417–425 (2009) UDC 633.822+66.061:665.52/.54 JSCS–3843 Original scientific paper
Supercritical CO2 extraction of mentha (Mentha piperita L.) at different solvent densities
ZORAN ZEKOVIĆ1*#, ŽIKA LEPOJEVIĆ1#, SLAVICA MILIĆ1, DUŠAN ADAMOVIĆ2 and IBRAHIM MUJIĆ3
1University of Novi Sad, Faculty of Technology, Department of Biotechnology and Pharma- ceutical Engineering, Bulevar Cara Lazara 1, 21000 Novi Sad, 2Institute of Field and Vegetable Crops, Maksima Gorkog 30, 21000 Novi Sad, Serbia and 3University of Bihać, Biotechnical Faculty, Kulina Bana 2, 77000 Bihać, Bosnia and Herzegovina
(Received 20 May, revised 28 October 2008)
Abstract: The chemical composition of mentha essential oil and mentha ex- tracts obtained at different pressures/temperatures by supercritical fluid extrac- tion (SFE) were studied by GC–MS. The menthol content was also determined spectrophotometrically. The predominant compounds in the essential oil and in the CO2 extract obtained at 100 bar were L-menthon and menthole but at higher pressures (from 150 to 400 bar), squalene was dominant. The equation of Naik et al. was used for modelling the mentha–supercritical CO2 system.
Keywords: Mentha piperita L.; essential oil; supercritical fluid extraction; ex- traction pressure and temperature; GC–MS.
The Labiatae family has several members with significant essential oil con- tents. Mentha essential oil is an important material for perfumery, as a flavour, for liquors, i.e., in cosmetics, in toothpastes, as well as spices in the food indus- try. Parts of the mint-family plant, mainly dry leaves, are used for tea worldwide. Commercial oils could be classified by their menthol or carvone content.1–3
The classical procedures for the separation of the active substances from plant material, i.e., steam distillation and extraction with organic solvents (e.g., dichloromethane) have serious drawbacks. The distillation procedure allows only the separation of volatile compounds (essential oils), which, to a greater or lesser extent, are transformed under the influence of the elevated temperature. On the other hand, extraction with organic solvents can hardly render an extract free of traces of the organic solvent, which are undesirable for organoleptic and/or health reasons. In addition, organic solvents are insufficiently selective, hence, in addi-
* Corresponding author. E-mail: email@example.com # Serbian Chemical Society member.
2009 Copyright (CC) SCS
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